Abstracts concerning M
نویسندگان
چکیده
In February 2000, Becton Dickinson Biosciences initiated a study to evaluate a potential new product, Herrold's Egg Yolk Agar with Mycobactin J and arnphotericin B, nalidixic acid, and vancomycin (BBL HEYA-MANV). The study involved laboratories which produced their own similar product, and compared the ability of the two media to support the growth of Mycobacterium avium subsp. paratuberculosis while suppressing contaminating overgrowth of fungi or bacteria. Fecal samples which the laboratories received for Johne's culture were processed according to the laboratory's routine procedure, inoculated onto both media, and evaluated for growth of M. avium subsp. paratuberculosis and contaminated at weekly intervals. Additionally, our laboratory inoculated tubes of BBL's Herrold's Egg Yolk Agar with Mycobactin J and amphotericin B (BBL HEYA), for purposes of comparing results to the two media listed above. Our hypothesis was that the additional antibiotics (nalidixic acid and vancomycin) in the media could suppress growth of M. avium subsp. paratuberculosis. The proportion of tubes with contaminating overgrowth was compared among media using the Pearson chi square test. Mean colony counts for the tubes with M. avium subsp. paratuberculosis growth were compared among media using the Kruskal-Wallis test.
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تاریخ انتشار 2001